Zeiss MP PHOTOMANIPULATION
Zeiss MP PHOTOMANIPULATION
2-photon Microscope for Photomanipulation
ROOM A204CN
Confocal laser scanning acquisition
Visualization of cells, organoids and tissue fragments
2-photon excitation - low phototoxicity and high depth of visualization: up to 2 mm
Second Laser for Photomanipulation: Uncaging, Photoconversion, Bleaching, Ablation etc.
Z-stacks, time series and their combinations
Colocalization and histogram analysis with individual parameters, number & brightness analysis, profile measurement along user-defined lines, measurement of lengths, angles, areas, intensities and much more
F-techniques: Forster Resonance Energy Transfer (FRET-AB)
Photobleaching techniques: FRAP (Fluorescence Recovery After Photobleaching), FLIP (Fluorescence Loss In Photobleaching)
Microscope
Upright Zeiss Axio Examiner Z1
Heated sample holder
Objectives
EC-PLAN-NEUFLUAR 10x/0.3 M27
Plan-APOCHROMAT 20x/1.0 W
Excitation
Coherent Chameleon Pulsed Laser 690-1064 nm
Coherent Chameleon M (Manipulation) Pulsed Laser 690-1064 nm
Detection
Camera AxioCam MRc 5
4x LSM BiG GaAsP detectors
Wide-field Filters
DAPI
GFP
DsRED
Confocal Filters
BP 420-480 + BP 500-550
BP 460-500 + BP 520-560
BP 500-550 + BP 570-610
405/150 BrightLine HC
488/50 BirghtLine HC
520/60 + 585/40 BrightLine HC
531/46 + 600/52 BrightLine HC
675/67 BrightLine HC
650/100 BrightLine HC
Samples
Standard microscopy slides with glass coverslip 75 x 26 mm
Tissue fragments and organoids
Cells