Zeiss LSM800 Airyscan
Zeiss LSM800 Airyscan
Confocal Laser Scanning Microscope with Airyscanning
ROOM 18 (ground floor)
Confocal laser scanning acquisition
AiryScan Super-Resolution module (1.4x better XYZ resolution)
Z-stacks, time series and their combinations
Colocalization and histogram analysis with individual parameters, number & brightness analysis, profile measurement along user-defined lines, measurement of lengths, angles, areas, intensities and much more
F-techniques: Forster Resonance Energy Transfer (FRET-AB, FRET-SE)
Photobleaching techniques: FRAP (Fluorescence Recovery After Photobleaching), FLIP (Fluorescence Loss In Photobleaching)
Laser-based transmitted light imaging
Microscope:
Inverted Zeiss Axio Observer
Manual stage
Objectives:
Plan Fluo 10x/0.3 DRY
Plan Apochromat 40x/1.40 Oil
Plan Apochromat 63x/1.40 Oil (Airyscan)
Diode lasers:
405 nm
488 nm
561 nm
640 nm
Detection:
2 PMT detector
Airyscan detector consisted of 32 GaAsP elements
Transmitted Light detection
Samples:
Standard microscopy slides with glass coverslip 75 x 26 mm
35 mm Cell Culture dishes
Lab-Tek™ Chambered coverslip/slide
IBIDI Chamber Slide/Coverslip