Zeiss Cell Discoverer 7 + LSM900 Airyscan 2
ZEISS CELL DISCOVERER 7
+ LSM 900 AiryScan 2
+ LSM 900 AiryScan 2
Automated Wide-field and Laser Scanning Confocal Microscope with Airyscanning
ROOM 214CN
Confocal Laser Scanning acquisition
Wide-Field Fluorescence Imaging
Transmitted light: Bright Field, PCG contrast
AiryScan 2 Super-Resolution module (1.4x better XYZ resolution, for live cells)
Z-stacks, time series, tile scan (mosaic), positions (mark & find) and their combinations
Colocalization and histogram analysis with individual parameters, number & brightness analysis, profile measurement along user-defined lines, measurement of lengths, angles, areas, intensities and much more
F-techniques: Forster Resonance Energy Transfer (FRET-AB, FRET-SE)
Photobleaching techniques: FRAP (Fluorescence Recovery After Photobleaching), FLIP (Fluorescence Loss In Photobleaching)
Long-term Live cell imaging (temperature and CO2 control)
Pippeting Port
Autofocus (Contrast-based, Reflection-based)
Advanced sample preview, multiwell sample navigation and advanced tile scan and positions features
Microscope
Inverted Zeiss Cell Discoverer 7
Motorized stage
Enviromental chamber with temperature and CO2 controler
Objectives
PLAN-APO 5x/0.3 DRY
PLAN-APO 20x/0.7 DRY
PLAN-APO 20x/0.95 DRY
PLAN-APO 50x/1.2 WATER Auto-immersion
Excitation
Diode Lasers (Confocal Mode):
- 405 nm
- 488 nm
- 561 nm
- 640 nm
Fluorescence Diodes (Wide-field Mode):
- 385 nm
- 420 nm
- 470 nm
- 511 nm
- 567 nm
- 590 nm
- 625 nm
Detection
Confocal Mode:
2 GaAsP Detectors
1 AiryScan 2 Detector
Wide-field Mode
Camera Axiocam 506
Emission Filterwheel:
QBP 425/30 + 514/30 + 592/25 + 709/100
TBP 467/24 + 555/25 + 687/145
TBP 425/30 + 524/50 + 688/145
Samples
Standard microscopy slides with glass coverslip 75 x 26 mm
35 mm Cell Culture dishes
Lab-Tek™ Chambered coverslip/slide
IBIDI Chamber Slide/Coverslip
Multiwell plate (live)