Zeiss Cell Discoverer 7 + LSM900 Airyscan 2

ZEISS CELL DISCOVERER 7
+ LSM 900 AiryScan 2

Automated Wide-field and Laser Scanning Confocal Microscope with Airyscanning

ROOM 214CN

Confocal Laser Scanning acquisition
Wide-Field Fluorescence Imaging
Transmitted light: Bright Field, PCG contrast
AiryScan 2 Super-Resolution module (1.4x better XYZ resolution, for live cells)
Z-stacks, time series, tile scan (mosaic), positions (mark & find) and their combinations
Colocalization and histogram analysis with individual parameters, number & brightness analysis, profile measurement along user-defined lines, measurement of lengths, angles, areas, intensities and much more
F-techniques: Forster Resonance Energy Transfer (FRET-AB, FRET-SE)
Photobleaching techniques: FRAP (Fluorescence Recovery After Photobleaching), FLIP (Fluorescence Loss In Photobleaching)
Long-term Live cell imaging (temperature and CO2 control)
Pippeting Port
Autofocus (Contrast-based, Reflection-based)
Advanced sample preview, multiwell sample navigation and advanced tile scan and positions features

Microscope

Inverted Zeiss Cell Discoverer 7
Motorized stage
Enviromental chamber with temperature and CO2 controler

Objectives

PLAN-APO 5x/0.3 DRY
PLAN-APO 20x/0.7 DRY
PLAN-APO 20x/0.95 DRY
PLAN-APO 50x/1.2 WATER Auto-immersion

Excitation

Diode Lasers (Confocal Mode):

- 405 nm

- 488 nm

- 561 nm

- 640 nm

Fluorescence Diodes (Wide-field Mode):

- 385 nm

- 420 nm

- 470 nm

- 511 nm

- 567 nm

- 590 nm

- 625 nm

Detection

Confocal Mode:
- 2 GaAsP Detectors
- 1 AiryScan 2 Detector
Wide-field Mode
- Camera Axiocam 506
- Emission Filterwheel:
  - QBP 425/30 + 514/30 + 592/25 + 709/100
  - TBP 467/24 + 555/25 + 687/145
  - TBP 425/30 + 524/50 + 688/145

Samples

Standard microscopy slides with glass coverslip 75 x 26 mm
35 mm Cell Culture dishes
Lab-Tek™ Chambered coverslip/slide
IBIDI Chamber Slide/Coverslip
Multiwell plate (live)

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