PicoQuant Symphotime 64

General Features :

• • Time gating

  • Binning

  • TCSPC Binning

  • TCSPC Fitting (Multi–Exponential Decay (1 to 5 Exponentials), Least–Squares Fitting, MLE Fitting, IRF Reconvolution, Tailfit, Bootstrap error analysis)

  • Global analysis of TCSPC curve fitting

  • GUI Themes 

Imaging :

• • FLIM, FLIM-FRET, Intensity FRET, Anisotropy Imaging, (Time-Gated) Fluorescence Intensity Imaging

  • Pattern Matching, Fast Pattern Matching

  • Adjustable color scale

  • Region-of-Interest (ROI)

  • Bin export for phasor analysis (via third party software Globals developed by the Laboratory for Fluorescence Dynamics) 

Correlation 

• • FCS, FCCS, FLCS, PIE–FCS

  • STED-FCS, STED-FLCS

  • FCS Fitting (Models: Diffusion Constants, Triplet state, Conformational, Protonation, Gaussian PSF, User-defined models via scripting, Bootstrap error analysis)

  • Global analysis

  • FCS calibration

  • Antibunching/Coincidence correlation, Total correlation 

FRET :

• • PIE (Pulsed Interleaved Excitation)

  • Bleedthrough Correction

  • FLIM-FRET