Zeiss MP IN VIVO
Zeiss MP7 In Vivo
In Vivo 2-photon Microscope
ROOM A204CN
Confocal laser scanning acquisition
Visualization of living small animals (mice, rats) via cranial window, cells, organoids and tissue fragments
2-photon excitation - low phototoxicity and high depth of visualization: up to 2 mm
Z-stacks, time series and their combinations
Colocalization and histogram analysis with individual parameters, number & brightness analysis, profile measurement along user-defined lines, measurement of lengths, angles, areas, intensities and much more
F-techniques: Forster Resonance Energy Transfer (FRET-AB)
Photobleaching techniques: FRAP (Fluorescence Recovery After Photobleaching), FLIP (Fluorescence Loss In Photobleaching)
Microscope
Upright Zeiss Axio Examiner Z1
Head holder for small animals
Objectives
EC-PLAN-NEOFLUAR 10x/0.3 M27
Plan-APOCHROMAT 20x/1.0 W
Excitation
Coherent Chameleon Pulsed Laser 690-1040 nm
OPO module 1050-1300 nm
Detection
2x LSM BiG GaAsP detectors
Wide-field Filters
DAPI
GFP
DsRED
Confocal Filters
BP 420-480 + BP 500-550
BP 460-500 + BP 520-560
BP 500-550 + BP 570-610
Samples
Standard microscopy slides with glass coverslip 75 x 26 mm
Living small animals with cranial window
Tissue fragments and organoids
Cells