Nanolive 3D Cell Explorer - fluo
Nanolive CX-A
Holotomography System
A207CN
Marker-free, 3D imaging of live cells
Measurements of Refractive Index (RI) of the sample in seconds and 3D
Wide-Field Fluorescence Imaging
Transmitted light: Bright Field
Z-stacks, time series, tile scan (mosaic), positions (mark & find) and their combinations
Live cell imaging (temperature and CO2 control)
Spatial resolution:
Holotomography: 200 nm (XY); 400 nm (Z)
Epifluorescence: 400 nm (XY), 2D only
Time resolution:
Holotomography: 0.5 fps 3D RI frame
Epifluorescence: 3 fps each channel
Microscope
Inverted Nanolive CX-A
Motorized stage
Objectives
60x DRY 0.8 NA
Excitation
520 nm low-power laser for RI measurements
CoolLED pE-300ultra for epifluorescence
Detection
CMOS Sony IMX174 camera
Epifluorescence DAPI/FITC/TRITC
Samples
Special glass-bottom, low-wall 96-Multiwell plates
Glass-bottom 35 mm Cell Culture dishes