Zeiss LSM780
Zeiss LSM780
Confocal laser scanning microscope
ROOM A211CN
Confocal laser scanning acquisition
Z-stacks, time series, spectral images, tile scan (mosaic), positions (mark & find) and their combinations
Fast spectral detection available (Lambda mode)
Colocalization and histogram analysis with individual parameters, number & brightness analysis, profile measurement along user-defined lines, measurement of lengths, angles, areas, intensities and much more
F-techniques: Forster Resonance Energy Transfer (FRET-AB, FRET-SE), FCS (Fluorescence Correlation Spectroscopy)
Photobleaching techniques: FRAP (Fluorescence Recovery After Photobleaching), FLIP (Fluorescence Loss In Photobleaching)
Live cell imaging (temperature and CO2 control) for selected sample types
Laser-based transmitted light imaging
Microscope
Inverted Zeiss Axio Observer Z.1
Motorized stage
Environmental chamber with temperature and CO2 controller
Objectives
EC Plan-Neofluar 10x/0.30 DRY
PL APO 20x/0.8 DRY
C-Apochromat 40x/1.2 W Corr FCS
Plan Apochromat 40x/1.4 Oil DIC
Plan Apochromat 63x/1.4 Oil DIC
Excitation
HeNe laser 633 nm
DPSS diode laser 561 nm
Argon laser:
458 nm
488 nm
514 nm
Diode laser 405 nm (CW/pulsed)
UV laser 355 nm
Detection
2 PMT detectors
1 GaAsP spectral detector (32 channels)
1 Transmitted Light detector
Samples
Standard microscopy slides with glass coverslip 75 x 26 mm
35 mm Cell Culture dishes
Lab-Tek™ Chambered coverslip/slide
IBIDI Chamber Slide/Coverslip
Multiwell plates