ONI Nanoimager S
ONI Nanoimager S
Super-resolution Microscope (SMLM) + TIRF/HiLo Microscope
ROOM A207CN
2D and 3D single-molecule localization microscopy (SMLM)
3D single-particle tracking (SPT)
Total Internal Reflection Fluorescence (TIRF)
Förster Resonance Energy Transfer (FRET)
Spatiotemporal resolution:
Up to 20 nm in XY, 50 nm in Z via SMLM
Up to 10 ms / 100 Hz with SPT, FRET, TIRF
Acquisition Speed:
100 fps full frame
2500 fps with frame height cropped to 2%
Wide-Field Fluorescence Imaging
Field of view: 50μm x 80μm
Autofocus (Reflection based)
Time Drift Correction
Sample preview
Microscope
Inverted ONI Nanoimager S
Motorized XYZ stage
Enviromental chamber with temperature controler
Objectives
100x / 1.4 NA OIL
Excitation
Lasers
405 nm
488 nm
561 nm
640 nm
Detection
sCMOS camera
100 fps full frame
2500 fps with frame height cropped to 2%
Compatible Samples
Standard microscopy slides with glass coverslip 75 x 26 mm
35 mm Cell Culture dishes
Lab-Tek™ Chambered coverslip/slide
IBIDI Chamber Slide/Coverslip