LEICA STELLARIS 5 STED
LEICA STELLARIS 5 STED
Confocal laser scanning microscope
SUPERRESOLUTION STED MICROSCOPE
LIGHT SHEET Microscope
ROOM A210CN
Time series, z-stacks, spectral images, tile scan and combination of mentioned applications
With this spectral information, any dye can be optimally excited with minimum cross-excitation and specimen damage
Lambda Square Mapping
Colocalization and histogram analysis with individual parameters, number & brightness analysis, profile measurement along user-defined lines, measurement of lengths, angles, areas, intensities and much more
Fixed fluorescently stained samples
Sensitive Hybrid Detectors for dim fluorescence staining
TauSense - set of imaging tools based on fluorescence lifetime: semi-FLIM, Tau Separation, Tau Gating, Tau Contrast, Tau STED
Superresolution STED microscopy
Digital Light Sheet microscopy
Microscope:
Inverted Leica DMI8
Motorized stage
Objectives:
SemiPlanApo 2,5x / 0.07 Dry
PlanApo 20x / 0.75 Dry
PlanApo 100x / 1.4 Oil STED
Excitation:
White light laser 485-685nm
Diode laser 405nm
Detection:
3 internal Power HyD (2 x HyD S oraz 1 x HyD X).
1 Transmited Ligh detector (TLD)
Spectral range of detector optics: 380 - 850nm
Samples:
Standard samples with glass coverslip
Culture dishes 35 mm (fixed cells)
Lab-Tek™ Chambered coverslip (fixed cells)
IBIDI Chamber Slide (fixed cells)
STED
Tau-STED 3X
Objective: PlanApo 100x / 1.4 Oil STED
XY resolution up to 30nm and Z resolution up to 100nm
775 deplition laser
Digital Lightsheet
Objective: PlanApo 10x / 0,30 WD = 3,6 mm Water Immersion
Light plane width 5mm
Camera: Hamamatsu Orca Flash 4.0 V3
Laser lines: 405nm, 488nm, 561nm, 636nm