ROOM 18
High-precision inverted research microscope system designed for advanced confocal imaging, super-resolution microscopy and next-generation MINFLUX nanoscopy, offering exceptional flexibility from routine fluorescence imaging to cutting-edge nanometer-scale localization
Advanced scanning system based on QUADScan technology enabling flexible ROI definition, arbitrary rotation, XYZ scanning and advanced imaging geometries (XZ/YZ planes)
Hybrid detector architecture combining single-photon avalanche detectors (APDs) with MATRIX spectral detection units for ultra-sensitive signal acquisition and background suppression
MATRIX detector technology enables time-resolved detection, improved signal-to-noise ratio and advanced pixel reassignment and deconvolution-based signal enhancement
RAYSHAPE deformable mirror-based adaptive optics system providing real-time correction of optical aberrations across all imaging depths without mechanical objective adjustment. Ensures optimal resolution and signal quality in thick specimens such as tissues, organoids and whole organisms, particularly beneficial for 3D-STED and deep confocal imaging
Time Correlated Single Photon Detection electronics and software. Synchronization unit between Picoquant and Abberior Instruments for simultaneous multi color lifetime recording
Time-resolved fluorescence imaging via TCSPC electronics enabling FLIM and photon arrival-based analysis with high temporal resolution. TIMEBOW software integrates fluorescence lifetime data with spectral and spatial information, enabling phasor-based analysis and lifetime-enhanced super-resolution imaging
2D and 3D STED imaging with resolution down to sub-100 nm in volume and ~30 nm lateral resolution depending on sample and fluorophore
MINFLUX Nanoscopy enabling nanometer-scale resolution
DIC observation method for a 60x objective lens
Fully integrated environmental control system enabling long-term live-cell imaging with regulated temperature, humidity and CO₂ conditions, as well as optional cooling control for sensitive samples
Microscope:
Inverted Olympus IX83
Motorized stage
200μm piezo z-stage
Okolab Bold Line Incubation and Cooling System
Z-drift Compensation Unit for Confocal and STED
Objectives:
UPLXAPO4X, 4x / NA 0.16 DRY
UPLXAPO20X, 20x / NA 0.8 DRY
UPLXAPO40XO, 40x / NA 1.40 OIL
UPLXAPO60xO, 60x / 1.42, OIL
UPLSAPO60XW, 60x / NA 1.2, W
UPLSAPO60XS, 60x / NA 1.3, SILICONE OIL
Excitation:
CW laser 405 nm
CW MINFLUX laser 640 nm
Pulsed lasers:
440 nm
485 nm
518 nm
561 nm
640 nm
685 nm
STED deplicion lasers:
589 nm
775 nm
Detection:
3 x APDs
2 x MATRIX
PMT detector for transmitted light
Spectral range of detector optics: 400 - 800 nm
Samples:
Standard samples with glass coverslip
Culture dishes 35 mm (fixed cells)
Well plates