Leica TCS SP8
LEICA TCS SP8
Confocal Laser Scanning Microscope
ROOM A210CN
Confocal laser scanning acquisition
Z-stacks, time series, spectral images, tile scan (mosaic), positions (mark & find) and their combinations
WLL (White Light Laser) 470-670 nm for precise adjustment of excitation wavelength
Spectral detection available (Lambda-scan)
Colocalization and histogram analysis with individual parameters, number & brightness analysis, profile measurement along user-defined lines, measurement of lengths, angles, areas, intensities and much more
F-techniques: FLIM (Fluorescence Lifetime Imaging), Forster Resonance Energy Transfer (FRET-FLIM, FRET-AB, FRET-SE), FCS (Fluorescence Correlation Spectroscopy)
Photobleaching techniques: FRAP (Fluorescence Recovery After Photobleaching), FLIP (Fluorescence Loss In Photobleaching)
Live cell imaging (temperature and CO2 control) for selected sample types
Navigator Module for advanced sample preview, multiwell sample navigation and advanced tile scan and positions features
Laser-based transmitted light imaging
Microscope
Inverted Leica DMI6000
Motorized XYZ stage
Enviromental chamber with temperature and CO2 controler
Objectives
HCX PL APO CS 10x/0.40 DRY
HC PL APO CS2 20x/0.70 Imm
HC PL APO CS2 40x/1.30 Oil
HC PL APO CS2 63x/1.20 Water
HC PL APO CS2 63x/1.40 Oil
Excitation
White Light Laser 470-670 nm (pulsed)
Argon laser (CW):
458 nm
476 nm
488 nm
496 nm
514 nm
Diode laser 405 nm (pulsed)
Detection
3 internal PMT detectors
2 internal HyD detectors
1 Transmited Light detector (TLD)
2 External Avalanche Photodiodes (SPAD)
Detector Ranges:
Internal: 380-800 nm
External:
470-550 + 607-683
422-448 + 500-550
467-499 + 535-585
500-550 + 565-605
500-530 + 607-683
500-550 + 547-703
500-550 + 565-605
Compatible Samples
Standard microscopy slides with glass coverslip 75 x 26 mm
35 mm Cell Culture dishes
Lab-Tek™ Chambered coverslip/slide
IBIDI Chamber Slide/Coverslip
Multiwell plates