LEICA AF 7000
Leica AF 7000
Fluorescence Microscope
ROOM 18 (ground floor)
Transmitted light and transmitted light contrast techniques (DIC, POL)
Widefield fluorescence visualization
Time series, z-stacks, tile scans and combination of mentioned applications
Calcium ion concentration analysis [FURA2]
Long-term live cells experiments (CO2 module temporarily unavailable)
Interference Reflection Microscopy (IRM) module for cell adhesion imaging
Autofocus (Contrast-based)
Microscope:
Inverted Leica DMI6000
Motorized XYZ stage
Enviromental Chamber with temperature and CO2 controler (temporarily unavailable)
Objectives:
PL FLUO 5x/0.3 DRY
HC PL FLUOTAR 10x/0.40 DRY
HC PL APO 20x/ 0.7 DRY
HC PL APO 40x/0.7 DRY
HCX PL APO 100x/1.40 Oil CS UV
HCX PL APO 100x/1.40 Oil CS
Excitation:
Fluorescence lamp
Filter cubes:
FU2 (Fura2)
I3 (GFP)
N2.1 (RFP)
Y5 (Far Red)
IRM (Reflection)
Detection:
Camera ANDOR DU8285_VP
Camera LEICA DCF 35DFXR2
Samples:
Multiwell plate (live experiments)
Standard samples with glass coverslip
Culture dishes 35 mm
Lab-Tek™ Chambered coverslip
IBIDI Chamber Slide