LEICA AF 7000

Leica AF 7000

Fluorescence Microscope

ROOM 18 (ground floor)

Transmitted light and transmitted light contrast techniques (DIC, POL)
Widefield fluorescence visualization
Time series, z-stacks, tile scans and combination of mentioned applications
Calcium ion concentration analysis [FURA2]
Long-term live cells experiments (CO2 module temporarily unavailable)
Interference Reflection Microscopy (IRM) module for cell adhesion imaging
Autofocus (Contrast-based)

Microscope:

Inverted Leica DMI6000
Motorized XYZ stage
Enviromental Chamber with temperature and CO2 controler (temporarily unavailable)

Objectives:

PL FLUO 5x/0.3 DRY
HC PL FLUOTAR 10x/0.40 DRY
HC PL APO 20x/ 0.7 DRY
HC PL APO 40x/0.7 DRY
HCX PL APO 100x/1.40 Oil CS UV
HCX PL APO 100x/1.40 Oil CS

Excitation:

Fluorescence lamp

Filter cubes:

FU2 (Fura2)
I3 (GFP)
N2.1 (RFP)
Y5 (Far Red)
IRM (Reflection)

Detection:

Camera ANDOR DU8285_VP
Camera LEICA DCF 35DFXR2

Samples:

Multiwell plate (live experiments)
Standard samples with glass coverslip
Culture dishes 35 mm
Lab-Tek™ Chambered coverslip
IBIDI Chamber Slide

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