Sample Preparation

Below you will find some crucial information how to prepare your samples in order to avoid microscope damage.

1. Waiting time after mouting the samples

It is strictly forbidden to observe freshly mounted samples on microscopes in the Facility. 

Minimal waiting time before observation:

• Non-curable mounting medium/buffer + Nail Polish: >16 hrs

• Curable medium: >16 hrs

• Addition-curing silicone (eg. picodent twinsil): >30 min

If you have doubts, don't hesitate to ask!

2. Coverslip placement

Incorrect coverslip placement makes it impossible to reach certain parts of the sample with high-magnification objectives and tilts the sample.

Observe the scheme below: