Zeiss Cell Observer Spinning Disc
Zeiss Cell Observer Spinning Disc
Spinning Disc Confocal Microscope
ROOM A214CN
Fast, gentle confocal acquisition
Z-stacks, time series, spectral images, tile scan (mosaic), positions (mark & find) and their combinations
Live cell imaging (temperature and CO2 control) for selected sample types
F-techniques: Forster Resonance Energy Transfer (FRET-SE)
Simultaneous detection of two channels using two cameras
Colocalization and histogram analysis with individual parameters, number & brightness analysis, profile measurement along user-defined lines, measurement of lengths, angles, areas, intensities and much more
Advanced sample preview, multiwell sample navigation and advanced tile scan and positions features
Autofocus (Reflection-based)
Microscope
Inverted Zeiss Axio Observer Z.1
Motorized stage
Enviromental chamber with temperature and CO2 controler
Objectives
PLN APO 10x/0.45 Dry
PL APO 20x/0.8 DRY
C APO 40x/1.20 Water
HC APO 63x/1.20 Water
PLN APO 63x/1.40 Oil
Excitation
Diode lasers:
405 nm
488 nm
561 nm
639 nm
Detection
2x Camera Photometrics Evolve 512
Emission Filters:
FF01 442/46 nm (blue)
FF01 457/50 nm (blue)
BP 525/50 nm (green)
BP 535/30 nm (green)
BP 600/50 nm (red)
FF01 697/58 nm (far red)
Samples
Standard microscopy slides with glass coverslip 75 x 26 mm
Culture dishes 35 mm (live experiments)
Lab-Tek™ Chambered coverslip
IBIDI Chamber Slide