ANDOR DSD 2
ANDOR DSD 2
Wide-Field Fluorescent Microscope with Confocal Module
ROOM 18 (ground floor)
Confocal Mode (Structured Illumination)
Wide-Field Fluorescence Imaging
Z-stacks, time series, tile scan (mosaic), positions (mark & find) and their combinations
Czy jest transmitted light???
Microscope:
Inverted Leica DMI6000
Motorized stage
Objectives:
N PLAN 10x/0.25 Dry
N PLAN L 20x/0.40 Dry
HC PL APO CS2 20x/0.70 Imm
HCX PL APO 40x/0.75 Dry
HCX PL APO 63x/1.40 Oil
Excitation:
Fluorescence Lamp
oświetlacz do confocal mode???
Structured illumination patterns (Confocal Mode):
high sectioning (highest resolution),
medium sectioning (medium)
high signal (lowest resolution)
Detection:
Camera Andor Zyla 5.5 sCMOS (Native resolution 2560x2160)
Filter cubes:
DAPI 390/40 Ex, 405 DM, 452/45 Em
GFP/FITC 482/18 Ex, 488 DM, 525/45 Em
RFP/DsRED 561/14 Ex, 561 DM, 609/54 Em
Cy5 640/14 Ex, 635 DM, 676/29 Em
Samples:
Standard microscopy slides with glass coverslip 75 x 26 mm
Culture dishes 35 mm with fixed cells
Lab-Tek™ Chambered coverslip
IBIDI Chamber Slide